ANXIOLYTIC EFFECT OF THE TUFTSIN ANALOGUE ON MICE WITH DIFFERENT HEREDITARY TYPES OF EMOTIONAL STRESS REACTION

M.M. Kozlovskaya 1, S.B. Seredenin 1, Y.A. Blednov 1, R. Czabak-Garbacz 2 and I.I. Kozlovsky 1

1 Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow, Russia
2 Department of Human Physiology of Medical Academy, Lublin, Poland

In our experiments the tuftsin analogue (heptapeptide, including Pro-Gly-Pro in its structure), enzimatic degradation-resistant, in single injection in wide interval of the doses (from 100 to 3000 mg/kg) on the male mice with two different hereditary types of emotional stress reaction - "passive" (Balb/c) and "active" (C57Bl/6) was administered. It produced in various stress situations, "unescapable" stress (open-field test) and "escapable" stress (elevated plus maze test and light-dark test), the inversion of emotionally-behaviour reactions on mice with high level of fear (Balb/c). The tuftsin analogue statistically significantly removed fear-manifestations, at the same time passive emotional stress reaction changed in orientation-searching activity. It was shown, that anxiolitic effect of heptapeptide had dose-dependent character, but without development of the sedation and brake. So we could say that tuftsin analogue had selective anxiolitic activity and stimulated behaviour of the mice with genetically controlled "passive" type of emotional stress reaction (Balb/c). In the mice with "active" type of emotional stress reaction (strain C57Bl/6) the heptapeptide did not show this type of activity. The results show the possibility of the future use of tuftsin analogue as a selective anxiety-relieving agent.


Poster presented at Measuring Behavior '96, International Workshop on Methods and Techniques in Behavioral Research, 16-18 October 1996, Utrecht, The Netherlands